The Phasefocus Livecyte is an inverted system for live cell imaging. This microscope has Quantitative Phase Imaging (QPI, also known as Ptychography) a label-free approach that utilises relative phase shift information to produces images of cells and tissues with high contrast and high fidelity with low phototoxicity and photobleaching. The system is also equipped with widefield fluorescence capabilities for researchers that wishes to identify cells and cellular components with high specificity. The system has automated single cell tracking algorithms with integrated analysis suite to monitor the cell’s behaviour, morphology, and motility over time. A fully enclosed temperature-controlled incubation unit (adjustable from 28oC – 40oC) with a CO2 controlling, humidity and evaporation, ensure cells remain stable and viable over the imaging period (days-weeks).