Common Applications
Topographic Imaging: | A topographic image is obtained while scanning with the AFM and formed by scale color mapping of the height measured at every pixel of the scan. Advanced scanning modes limits the damage of very soft samples by an accurate control of the force set point (from pN to nN) as well as protecting the probe ensuring consistent, long-term, high-resolution imaging. |
Mechanical Properties on soft samples: | The AFM supports measurements in PeakForce QNM (Quantitative Nanomechanical Mapping), QI advanced mode and advanced force spectroscopy. QNM and QI allow for the simultaneous acquisition of multiple sample properties including topology, modulus, adhesion and deformation. Both features allow for the capture and analysis of individual force curves at each pixel of the image. These features have been used to image cells, tissues and biomaterials such as hydrogels. |
Optical microscopy correlation: | The Nanowizard AFM is positioned on a manual inverted widefield epifluorescence microscope (Nikon Ti-U2), allowing for a real time import and registration of optical images with the DirectOverlay™ 2 software. Optical images can be used to guide AFM imaging or force measurements and allow AFM and optical image overlay. |
Single molecules and membranes: | The AFM high resolution allows for the imaging and force spectroscopy of Proteins, other biomolecules, and bio molecular assemblies as well as lipid monolayers and bilayers. |
Cell-substrate / Cell-cell spectroscopy: | The long z range of the hybrid stage allows for cell-substrate and cell-cell force spectroscopy. A cell, chemically bound to a cantilever, is bought in contact with the sample of interest (molecular layer, single cell, biomaterial…). The user sets the time of contact and a force vs. distance curve is recorded while the cell is separated again, to determine adhesion, viscoelastic parameters, single molecule events as well as tether formation. |
Micro rheology: | The microrheology module performs oscillations in z while in contact with the surface, to probe the frequency dependent response of the surface. It is available in the ForceRamp Designer in Advanced Force spectroscopy mode. |