Assays and Techniques

PROTEOMICS

• Analysis of tryptic digests, gel-fractions and peptides in biological extracts
• Determine pancreatic polypeptide levels in human plasma
• Mass determinations of intact proteins/glysosylations level estimation
• Top/Middle Down protein analysis by ECD and ETD
• Development of phosphorylation assays used to investigate cell-signalling
• Phosphopeptide analysis by TiO2, IMAC and anti-phosphotyrosine enrichment
• Quantitation of specific peptides in biological fluids/tissues/organism by MRM
• Low mass peptide/protein (<25kDa) enrichment from complex proteomes
• nanoLC-MS/MS protein digest analysis (LysC, AspN, Chyotrypsin etc)
• De novo sequencing of peptides and small proteins
• Mascot, SEQUEST, MaxQuant or PEAKS searches for protein identification
• High resolution mass accuracy analysis (including ECD, ETD, IRMPD)
• iTRAQ analysis and quantitation
• SILAC analysis and quantitation
• High resolution UPLC label free protein quantification 
• Static nanospray of peptides and proteins including antibodies

METABOLOMICS

• Isotope dilution GC/MS or LC/MS assays
• Analysis of catecholamines
• Kynurenine Pathway metabolite identification
• Nitrates and Nitrites analysis
• Glucose quantification
• Hormone identification and quantification
• Acetylcholine and other quaternary ammonium biological amines
• Quantification of Vitamin D and metabolites
• Assay for the quantification of dityrosine in urine and protein hydrolysates
• Detection of lactones in microbial extracts
• Analysis of coal tar samples for molecular weight distribution
• Analysis of borate complex structure in plant extracts
• Metabolite profiling by LC-MS or GC-MS
• Untargeted metabolomics
• Orbitrap LC-MS for accurate mass small molecule analysis
• Triple quadrupole LC-MS for small molecule analysis
• Ion trap LC-UV-MS for small molecule analysis
• Small molecule quantitation by mass spectrometry
• Drug quantitation in biological fluids by mass spectrometry
• Targeted quantitation of specific molecules in biological fluids/tissues by mass spectrometry
• Folate quantitation

LIPIDOMICS

• Isotope dilution GC/MS or LC/MS quantification
• Lipid analysis or profiling by high resolution LC-MS and MS/MS
• Quantification of lipids by nanospray

IRMS

• Kiel/IRMS analysis of carbonate
• Elemental Analysis/IRMS

OTHER TECHNIQUES

• Analysis of synthetic polymers and large synthetic organometallics
• HPLC separations
• UPLC separations
• Surface analysis by DESI-Orbitrap
• nano-DSC
• nano-iTC
• Synthetic Biology.  Many of the analytical capabilities of the BMSF may be utilized in research area.