The Shimadzu Gel Permeation Chromatography (GPC) system is used to separate and give size distributions of polymers. The instrument uses THF as an isocratic eluent, and all samples must be dissolved in THF for use on this system.
Samples are separated using both a Phenogel 10u, 300 x 7.8 mm column amd a Phenogel 00H-0441-KO 5μm, 50Å 300 x 7.8 mm column in series fitted with a Phenogel 10µ guard column (Phenomenex, US). The instrument is capable of separating molecular weights of 150 to 1000000 Da.