Nikon AXR

Nikon AXR

 

The Nikon AX-R2 is a confocal system with 2k x 2k resonant scanning and 8k x 8k Galvano scanning technologies. The system is designed to balance sensitivity and resolution, with the capacity to perform spectral scanning and unmixing. The system is fitted with 4 detectors and 404/488/561/660nm lasers for a wide range of applications.
The system is supported by NIS-elements software with AI technology and conditional imaging modules built into Nikon JOBS. The AX-R2 system is built on the Nikon Ti2-E fully motorised inverted microscope. It has the largest field of view (25mm) on the market, enabling users to capture single-shot images of large samples. This results in less tiles to stitch and a reduction in imaging time. The range of objectives have Nano-coating technology for optimal performance.

Microscope body:
Inverted
Motorised Stage:
Yes
Temp Control:
Stage Insert
CO2:
Stage Insert
Software:
NIS-elements

 

Funded by Cancer Institute NSW

Common Applications

 

Confocal: Confocal imaging is a fluorescence microscopy technique that optically sections the specimens preventing out-of-focus light from reaching the detector. This yields clear high contrast images, and together with the ability to acquire images sequentially at multiple positions the entire sample can be reconstructed in 3D.
High-Speed Resonant Scans:

The combination of the large FOV with the AX-R2’s resonance scanner allows for high-speed imaging of large regions of interest. The fast acquisition allows users to capture dynamic events in live specimens in confocal mode with a high temporal resolution, and reduced imaging times for fixed samples

Automated image acquisition with real-time feedback

Using NIS-Elements with the JOBS module allows for customised multidimensional imaging with capabilities to set multipoint imaging, tiling, z-stacks, time series, multichannel, and both widefield and confocal imaging. Real-time analysis of data can be integrated and provide feedback to imaging acquisition. 

Spectral Unmixing The tuneable emission filter in the DUX-VB detector allows the user to remove unwanted emitted fluorescence. The system also allows for the acquisition of hyperspectral images in up to 66 emission channels for unmixing. 

 

Objective Lenses

MAG N.A CORRECTIONS IMMERSION W.D
10x 0.25 CFI Plan Apochromat Lamba S Air 7 mm
20x 0.75 CFI Plan Apochromat VC Air 1.0 mm
40x 0.95 CFI Plan Apochromat Lamba Air 0.21 mm
60x 1.3 CFI Plan Apochromat VCWater   0.31-0.28 mm

 

Light Sources

Pillar for transmitted illumination

LED lines: 385, 470, 525, and 625nm

Lasers: 405, 488, 561, and 640nm

Filters

For Epifluorescence

Name                    Excitation                            Dichroic                Emission

C-LED385             BP 371-409 nm                 LP 420 nm           BP 430-520 nm

C-LED470             BP 450-490 nm                 LP 500 nm           BP 506-562 nm

C-LED525             BP 500-550 nm                LP 560 nm           BP 568-626 nm

C-LED625             BP 592-650 nm                 LP 660 nm           BP 669-743 nm

 

For Confocal

The DUX-VB detector has a tuneable emission filter that can be match to the users’ fluorescent labels in NIS-Elements.

Detectors

DUX-VB4 DUVB 4Ch Detector Unit with 2 PMT-MA Multi Alkali Units and 2 PMT-GA GaAsP Units

DS-Qi2 Monochrome Microscope Camera

Miscellaneous

Perfect focus, Resonant scanner, Phase objectives