| Lightsheet microscopy is a fluorescence-based imaging technique that is growing in popularity. It outperforms other techniques in its ability to rapidly capture large optically sectioned three dimensional datasets, whilst keeping photo-toxicity and bleaching to a minimum. It therefore lends itself well to imaging samples larger than those typically attempted by conventional fluorescence microscopy such as tissues, whole organs and even embryo development. |
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| Lightsheet microscopy | Lighsheet microscopy creates and positions a sheet of excitation light across the focal plane of a collection objective. In doing so it restricts excitation events in a particular plane of the sample resulting in an optically sectioned image drastically reducing light emanating from outside of the focal plane. Moving the sample through this plane allows rapid collection of sectioned 3D data. |
| Tissue Clearing: | Tissue clearing is a preparation technique with the aim of reducing the inherinatly scattering nature of tissue. There are several means by which this is achieved but all enable clearer and deeper penetration into the sample using light microscopy. |
| Live Cell Imaging | Lightsheet imaging can also be done in brightfield and/or fluorescence on a small live and fairly transparent specimen (e.g. Spheroids, Organoids, zebra fish …). The chamber can be heated to the temperature required and the specimen is immersed in its medium during the imaging. |
