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The 3i Lattice LightSheet allows for gentle 4D imaging of live cells. The system creates an ultra-thin Gauss-Bessel light sheet that can be used to image cells for short or long time periods. This system has high spatial and temporal resolution due to the unique lattice lightsheet. Images can be captured in excess of 500 frames per second. The custom-made illumination water immersion objective has a long working distance ideal for lightsheet illumination. The high-resolution imaging objective has an NA of 1.1 and is matched to the lightsheet thickness, enabling excellent optical sectioning at a subcellular level. These objectives remain fixed and aligned, allowing users to navigate their sample with the motorised stage. The solid-state heated stage has active feedback for thermal stability. The supporting software, SlideBook, enables rapid switching between exposures, wavelengths, and regions of interest, and is a streamlined control module for image acquisition. |
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Funded by Cancer Institute NSW
