Common Applications
| Spinning disk confocal | The Nikon TiE2 has a CSU-W1 spinning disk confocal unit. This allows for the high-speed acquisition of images with a high spatial optical resolution. The CSU-W1 can accommodate a wide field of view and has reduced pinhole crosstalk with the Nipkow disk. |
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| Live-cell imaging | Imaging living cells allows observation of dynamic processes, greatly improving our understanding of how biological mechanisms work. To facilitate cell survival and to simulate biologically relevant conditions for cellular function, this system includes a temperature and atmosphere controlled stage insert. This wide-field fluorescence system allows gentle imaging of live cells with minimal phototoxic impact. Further, Nikon’s NIS Elements software allows for easy setup of long-term live-cell experiments for visualisation of cell migration and cell proliferation. |
| Automated experiments | This system is equipped with Nikon’s JOBS software, providing a platform for setting up automated experiments. Using a modular interface, JOBS allows users to pre-set imaging parameters and perform long-term experiments that run without further input. In addition, an attached Cetoni liquid handling unit allows integration of buffer-exchange into automated imaging protocols for applications such as multiplexed imaging. |
