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The Zeiss Lattice Lightsheet 7 is an easy to use system for performing fast and gentle volumetric imaging of living cells, cellular organelles, and its structures. This inverted fluorescence system features automatic alignment of all optical elements, auto-immersion, time course, positions, and tiles to allow researchers to set up and leave long term experiments. The Lattice Lightsheet is made possible by combining lightsheet technology; the concept of decoupling the illumination and detection pathway together, with advanced beam shaping technology to create lattice-shaped light sheets. This allows high temporal resolution imaging where up to 3 volumes scan per second acquisition can take place with near-isotropic resolution in confocal range. A maximum of 400 frames per second can be attained on this system. This system is fitted with 13.3x/0.4 illumination objective and a 44.83x/1.0 detection objective. Lattice lightsheet resolution is defined by the beam length and thickness, there are six pre-defined sheets to choose from with axial resolution ranging from 500-1000 nm in deskewed datasets and 450-900nm deskewed plus deconvolution datasets. In addition, the system has inbuilt deskewing, cover glass transformation and deconvolution functions which can be incorporated into the main acquisition setup where deskewing, transformation and deconvolution can happen on the fly. This system operates using 488, 561 and 641 nm lasers, has a white or red LED for transmitted light and two Hamamatsu ORCA-Fusion cameras for fast simultaneous or sequential imaging. This imaging modality is well suited to capturing dynamic biological process in live samples at high volume speed, developing life at initial stages i.e. oocytes, embryo, c. elegans, spheroids, pollen grain and culture cells. |
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