Zeiss Apotome2

Zeiss Apotome2
The Zeiss Axio Imager M2 upright microscope with ApoTome 2 with MBF Bioscience software: Stereo Investigator and Neurolucida. This system can be used for neuronal tracing, morphometric quantification, and unbiased stereology (number, length, area and volume of cells and biological structure stereology of histological specimens) in both brightfield and fluorescence. The system is fitted with 6 objectives ranging from 2.5x to 63x, making it ideal for widefield imaging of cells or tissues. The Apotome 2 allows optical sectioning of fluorescent samples by the removal out of focus light with structured illumination (uses grid patterns to calculate the optical sections). The system has both monochrome and colour cameras.  
Microscope body: Upright
Motorised Stage: Yes
Temp Control: No
CO2: No
Software: Stereo Investigator and Neurolucida

Common Applications

Unbiased Stereology - Automated workflows for Apotome.2 optical fractionator, and for acquisition of multi-channel fluorescent 3D image stacks  
- Contour tracing and cell counting 
- Serial section manager and 2D Anatomical Mapping  
- Direct export of data & results to Microsoft Excel 
Neurolucida - 3D Neuron tracing & reconstruction 
- Brain Mapping & Anatomical Mapping  
- Quantitative Morphometric Analysis 
- 3D visualization of traced models (rotate & zoom) including adjusting surface transparency & lighting conditions, etc.  
- Automatic cell detection  
- Direct export of results to Microsoft Excel 

Objective Lenses

MAG

N.A.

CORRECTIONS

IMMERSION

 

W.D.

2.5x

0.085

EC- Plan NeoFluar

Air

 

8.8 mm

5x

0.16

EC- Plan NeoFluar

Air

 

18.5 mm

10x

0.45

Plan-Apochromat

Air

 

2.1 mm

20x

0.8

Plan-Apochromat

Air

 

0.55 mm

40x

0.95

Plan-Apochromat

Air

 

0.25 mm

63x

1.4

Plan-Apochromat

Oil

 

0.19 mm

Light Sources

Zeiss Colibri 7 

Transmitted light unit: LED 

Fluorescence: LEDs 385, 423, 469, 511, 555, 590, 631 and 735 

Filters

NAME 

EXCITATION 

DICHROIC 

EMISSION 

FS 96 HE

BP 370-410 nm  

LP 420 nm 

BP 430-470 nm 

FS 38 

BP 450-490 nm

LP 495 nm

BP 500-550 nm

FS 43

BP 532-558 nm

LP 570 nm

BP 570-640 nm

FS 50

BP 625-655 nm

LP 660 nm

BP 665-715 nm

FS 64 HE

BP 575-600 nm

LP 605 nm

BP 610-680 nm

Detectors

AxioCam 506c color digital camera 

Hamamatsu ORCA Flash 4.0 LT Plus USB3 & CamLink: monochrome digital camera 

Miscellaneous

Optical sectioning with Zeiss Apotome 2 

For offline analysis see Image Analysis Computers: computer MCP

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Authorised by the Executive Director of Mark Wainwright Analytical Centre, UNSW CRICOS Provider Code 00098G, ABN 57 195 873 179

Mark Wainwright Analytical Centre, UNSW, Sydney, NSW, 2052, Australia | Email: analytical@unsw.edu.au