Nikon A1 Intravital

The Nikon A1 intravital microscope is an inverted confocal system designed for fast stable imaging over time. With two scanners, a standard Galvano scanner for achieving images with a high signal-to-noise ratio and a resonant scanner for when speed is of upmost importance, this system is suitable for a wide range of experiments. Equipped with 4 dedicated PMTs for reflective/fluorescence imaging, 2 of which as high efficiency GaAsP detectors, and a hardware solution (Perfect Focus System) for preventing drift during imaging this system yields both high sensitiy with speed and stability. 

Fluorescence Recovery After Photobleaching is used to gain insight into the dynamics or both diffusion and binding rates within a sample. Photobleaching is an irreversible process by which a fluorophore losses the ability to absorb and emit light. By deliberately inducing photobleaching within a restricted region of the sample the observed recovery within this region over time is affected by the rate at which unbleached molecules entre this region and bleached molecules are removed.

Light microscopy lends itself very well to labelling multiple structures within a sample due to the ability to separate these spatially overlapping signals by the wavelength of light they emit. Colocalisation is the study of how the distribution of one probe relates to that of another within the same sample.