EasyGel And SmartClear II

To date there are two main approaches to clearing tissue (aqueous based and solvent based). Aqueous based clearing is further classified into three main classes:

1. Simple refractive index matching
2. Removal of lipids plus hyperhydration/refractive index matching.
3. Tissue-gel-hybridization plus refractive index matching.

The EasyGel and SmartClear II is a commercial system designed to assist in the tissue-gel-hybridization plus refractive index matching process. Samples that are produced under this method are known as CLARITY, PACTS, SWITCH etc. Tissue-gel-hybridization can be imaged using any light microscopy methods such as confocal, multiphoton and lightsheet.

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EasyGel

Tissue-gel-hybridization system (easy Clear)

Vacuum system – 0 to 70kPa

Temperature controlled – 20 to 50˚C, Individual slot temperature control

Shaking speed control 0-60 RPM

Holds 8 samples per run (50 ml Falcon tubes)

SmartClear II Standard system

Active clearing system uses stochastic electrotransport technology

Sample rotation speed – 0 to 10 RPM

Current or voltage control

Polarity direction change

Temperature controlled – 0 to 50˚C

Sample holders for tissue slices, different size of organs