Leica RM2135 Rotary Microtome

Sample paraffin blocks, single-use blades, conventional microscope slides, ice tray and water bath.

• Re-hydrate paraffin embedded specimen before cutting thin sections. Place the side to be cut on ice (block of ice in a tray) for at least 15 mins, up to a few hours depending of the dryness of the paraffin block.
• Prepare water bath (40-45^oC) ready to use.
• Place a blade or a single-use blade on blade holder, place the paraffin block into the block clamp and bring the block holder to its rear limit.
• Select a cutting thickness and orientate the block to the right position.
• Cut sections. If the sections are thin 4-5 um they make a ribbon of sections attached to each other. Pick up the ribbon delicately and spread it on top of warm water.
• Collect sections one by one with a microscope slide by lifting the slide while the section sticks to it.
• De-engage the brake, dispose of the single-use blade in sharp bin if required, and repeat the procedure with the next pre-cooled specimen.
• Clean up water bath and working area after the work is finish.