Common Applications
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High-Throughput |
The operetta is able to image up to 384 well per assay in a fully automated way |
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Live Cell Imaging |
Many biological processes are dynamic events and as such observing living cells is crucial to unravelling and understanding the mechanisms behind them. This requires special consideration when imaging. Firstly, the environmental conditions must be controllable and stable to ensure the cell remain healthy. Secondly, care needs to be taken that the act of imaging has as little impact as possible on the event being observed. |
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Spinning Disk Confocal |
Spinning Disk microscopy allows fast confocal-like optical sectioning. By utilising multiple pinholes in a rotating disk several points can be scanned in parallel enabling rapid acquisitions. Especially useful when trying to observe fast biological processes, such as vesicle transport in live cell imaging or thick optically cleared of small spheroids or organoids. |
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Colocalisation |
To Complete |
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Imaging of Cleared Samples |
Tissue Clearing is a preparation technique with the aim of reducing the inherently scattering nature of tissue. There are several means by which this is achieved but all enable clearer and deeper penetration into the sample using light microscopy. |
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FRET |
FRET (Fluorescence Resonance Energy Transfer) is a technique used largely to investigate molecular interactions, or with specially designed reports it can show if molecules are in an active or inactive state. It works by using two carefully selected fluorophores that when in close enough proximity and suitable orientation energy from the donor fluorophore can be transferred to the accepter. This transfer can be observed by monitoring the acceptors emission or the donor fluorescence lifetime. |
