Dr Daniel Luque is associate director of the Electron Microscopy Unit and senior lecturer at the School of Biomedical Sciences at UNSW. He holds a Ph.D. degree in Molecular Biology from the Autonoma University of Madrid, Spain. During his career, he has been devoted to the structural characterisation of macromolecular complexes using cryogenic electron microscopy techniques. He has over 20 years of experience in the structural analysis of biological samples through a broad methodology of microscopy techniques and has been directly involved in the implementation of different three-dimensional electron microscopy methodological developments. He has made significant contributions to the structural characterisation of complexes derived from a number of viral systems including ssRNA, dsRNA, ssDNA and dsDNA viruses. He has undertaken the structural characterisation of a wide range of macromolecular assemblies derived from these systems including the solution of >100 three-dimensional structures by electron microscopy.
Dr Daniel Luque
Associate Director
Facility:
Category: Academic Staff
Research profile: Click to view
Biography
My Research Activities
Dr. Luque research is focused on the structural characterisation of viral macromolecular complexes by three-dimensional electron microscopy techniques, specifically cryoEM and image processing. He have made significant contributions to the structural characterisation of a number of viral systems including i) dsRNA viruses such as Rotavirus, Birnavirus and several fungal viruses; ii) ssRNA viruses such as rabbit haemorrhagic disease virus, human rhinovirus, respiratory syncytial virus, human metapneumovirus, and cowpea chlorotic mottle virus; iii) ssDNA viruses such as beak and feather disease virus and bat circovirus and iv) dsDNA bacteriophages such as T5, T7 and P22.
The most recent focus of his research is investigating how different viruses interact with the complex membranous system that surround and reside within the cell. In collaboration with other groups he is studding this phenomenon in three main research lines: i) development of nanotransporters/nanocages based on viral capsids, ii) membrane fusion proteins from different viruses including human respiratory syncytial virus (hRSV), human metapneumovirus (hMPV) and coronavirus (SARS-CoV-2 & MERS) and iii) rotavirus entry and tropism.
Publications
Book Chapters
, 2023, 'Cryo-Electron Microscopy and Cryo-Electron Tomography of Viruses', in Physical Virology, Springer International Publishing, pp. 283 - 306, http://dx.doi.org/10.1007/978-3-031-36815-8_12
, 2019, 'Structural Insights into Rotavirus Entry', in Advances in Experimental Medicine and Biology, pp. 45 - 68, http://dx.doi.org/10.1007/978-3-030-14741-9_3
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, 2013, 'Chrysovirus Structure. Repeated Helical Core as Evidence of Gene Duplication', in Advances in Virus Research, pp. 87 - 108, http://dx.doi.org/10.1016/B978-0-12-394315-6.00004-0
, 2013, 'Chrysovirus Structure', in Advances in Virus Research, Elsevier, pp. 87 - 108, http://dx.doi.org/10.1016/b978-0-12-394315-6.00004-0
Journal articles
, 2024, 'The recruitment of TRiC chaperonin in rotavirus viroplasms correlates with virus replication', mBio, 15, http://dx.doi.org/10.1128/mbio.00499-24
, 2024, 'Recombinantly expressed virus-like particles (VLPs) of canine circovirus for development of an indirect ELISA', Veterinary Research Communications, 48, pp. 1121 - 1133, http://dx.doi.org/10.1007/s11259-023-10290-z
, 2024, 'Accumulation of endogenous free radicals is required to induce titan-like cell formation in Cryptococcus neoformans', mBio, 15, http://dx.doi.org/10.1128/mbio.02549-23
, 2023, 'Rotavirus Particle Disassembly and Assembly In Vivo and In Vitro', Viruses, 15, pp. 1750, http://dx.doi.org/10.3390/v15081750
, 2023, 'MicroRNA signature from extracellular vesicles of HCV/HIV co-infected individuals differs from HCV mono-infected', Journal of Molecular Medicine, 101, http://dx.doi.org/10.1007/s00109-023-02367-8
, 2023, 'Lippia origanoides Essential Oil or Thymol in Combination with Fluconazole Produces Damage to Cells and Reverses the Azole-Resistant Phenotype of a Candida tropicalis Strain', Journal of Fungi, 9, http://dx.doi.org/10.3390/jof9090888
, 2023, 'Equilibrium Dynamics of a Biomolecular Complex Analyzed at Single-amino Acid Resolution by Cryo-electron Microscopy: Equilibrium Dynamics Revealed by cryo-EM Maps', Journal of Molecular Biology, 435, http://dx.doi.org/10.1016/j.jmb.2023.168024
, 2022, 'Identification and Characterization of Epithelial Cell-Derived Dense Bodies Produced upon Cytomegalovirus Infection', Vaccines, 10, http://dx.doi.org/10.3390/vaccines10081308
, 2022, 'Erratum: Cryo-EM structures show the mechanistic basis of pan-peptidase inhibition by human α2 -macroglobulin (Proceedings of the National Academy of Sciences of the United States of America (2022) 119 (e2200102119) DOI: 10.1073/pnas.2200102119)', Proceedings of the National Academy of Sciences of the United States of America, 119, http://dx.doi.org/10.1073/pnas.2208467119
, 2022, 'Cryo-EM structures show the mechanistic basis of pan-peptidase inhibition by human α2 -macroglobulin', Proceedings of the National Academy of Sciences of the United States of America, 119, http://dx.doi.org/10.1073/pnas.2200102119
, 2022, 'Reply to Harwood et al.: Alternative functional conformations of native human α2 -macroglobulin', Proceedings of the National Academy of Sciences of the United States of America, 119, http://dx.doi.org/10.1073/pnas.2211048119
, 2021, 'Rotavirus Binding to Cell Surface Receptors Directly Recruiting α2 Integrin', Advanced NanoBiomed Research, 1, http://dx.doi.org/10.1002/anbr.202100077
, 2021, 'A small non‐coding rna modulates expression of pilus‐1 type in streptococcus pneumoniae', Microorganisms, 9, http://dx.doi.org/10.3390/microorganisms9091883
, 2021, 'Structural Perspectives of Beak and Feather Disease Virus and Porcine Circovirus Proteins', Viral Immunology, 34, pp. 49 - 59, http://dx.doi.org/10.1089/vim.2020.0097
, 2020, 'Cryo-electron Microscopy Structure, Assembly, and Mechanics Show Morphogenesis and Evolution of Human Picobirnavirus', Journal of Virology, 94, http://dx.doi.org/10.1128/JVI.01542-20
, 2020, 'Cryo-electron microscopy for the study of virus assembly', Nature Chemical Biology, 16, pp. 231 - 239, http://dx.doi.org/10.1038/s41589-020-0477-1
, 2020, 'Four-Dimensional Characterization of the Babesia divergens Asexual Life Cycle, from the Trophozoite to the Multiparasite Stage', mSphere, 5, http://dx.doi.org/10.1128/mSphere.00928-20
, 2019, 'Antiviral activity of aged green tea extract in model food systems and under gastric conditions', International Journal of Food Microbiology, 292, pp. 101 - 106, http://dx.doi.org/10.1016/j.ijfoodmicro.2018.12.019
, 2019, 'Structural nanotechnology: Three-dimensional cryo-EM and its use in the development of nanoplatforms for: In vitro catalysis', Nanoscale, 11, pp. 4130 - 4146, http://dx.doi.org/10.1039/c8nr09204d
, 2018, 'Kinetics of the invasion and egress processes of Babesia divergens, observed by time-lapse video microscopy', Scientific Reports, 8, http://dx.doi.org/10.1038/s41598-018-32349-7
, 2018, 'The RNA-binding protein of a double-stranded RNA virus acts like a scaffold protein', Journal of Virology, 92, http://dx.doi.org/10.1128/JVI.00968-18
, 2018, 'Cargo Retention inside P22 Virus-Like Particles', Biomacromolecules, 19, pp. 3738 - 3746, http://dx.doi.org/10.1021/acs.biomac.8b00867
, 2018, 'Capsid structure of dsRNA fungal viruses', Viruses, 10, http://dx.doi.org/10.3390/v10090481
, 2018, 'Biophysical properties of single rotavirus particles account for the functions of protein shells in a multilayered virus', eLife, 7, http://dx.doi.org/10.7554/eLife.37295
, 2018, 'Chimeric Pneumoviridae fusion proteins as immunogens to induce cross-neutralizing antibody responses', EMBO Molecular Medicine, 10, pp. 175 - 187, http://dx.doi.org/10.15252/emmm.201708078
, 2017, 'Acquisition of functions on the outer capsid surface during evolution of double-stranded RNA fungal viruses', PLoS Pathogens, 13, http://dx.doi.org/10.1371/journal.ppat.1006755
, 2017, 'Structural Characterization of Native and Modified Encapsulins as Nanoplatforms for in Vitro Catalysis and Cellular Uptake', ACS Nano, 11, pp. 12796 - 12804, http://dx.doi.org/10.1021/acsnano.7b07669
, 2017, 'XTEND: Extending the depth of field in cryo soft X-ray tomography', Scientific Reports, 7, http://dx.doi.org/10.1038/srep45808
, 2017, 'Assembling enzymatic cascade pathways inside virus-based nanocages using dual-tasking nucleic acid tags', Journal of the American Chemical Society, 139, pp. 1512 - 1519, http://dx.doi.org/10.1021/jacs.6b10948
, 2016, 'Cargo-shell and cargo-cargo couplings govern the mechanics of artificially loaded virus-derived cages', Nanoscale, 8, pp. 9328 - 9336, http://dx.doi.org/10.1039/c6nr01007e
, 2016, 'Influence of respiratory syncytial virus F glycoprotein conformation on induction of protective immune responses', Journal of Virology, 90, pp. 5485 - 5498, http://dx.doi.org/10.1128/JVI.00338-16
, 2016, 'Cell wall changes in amphotericin B-resistant strains from Candida tropicalis and relationship with the immune responses elicited by the host', Antimicrobial Agents and Chemotherapy, 60, pp. 2326 - 2335, http://dx.doi.org/10.1128/AAC.02681-15
, 2016, 'Structural insights into the assembly and regulation of distinct viral capsid complexes', Nature Communications, 7, http://dx.doi.org/10.1038/ncomms13014
, 2016, 'Ultrastructure of the Babesia divergens free merozoite', Ticks and Tick-borne Diseases, 7, pp. 1274 - 1279, http://dx.doi.org/10.1016/j.ttbdis.2016.07.001
, 2016, 'Engineering, Structure and Immunogenicity of the Human Metapneumovirus F Protein in the Postfusion Conformation', PLoS Pathogens, 12, http://dx.doi.org/10.1371/journal.ppat.1005859
, 2016, 'Heterodimers as the structural unit of the T=1 capsid of the fungal double-stranded RNA Rosellinia necatrix quadrivirus 1', Journal of Virology, 90, pp. 11220 - 11230, http://dx.doi.org/10.1128/JVI.01013-16
, 2015, 'Structural and functional insights into Escherichia coli α2 -macroglobulin endopeptidase snap-trap inhibition', Proceedings of the National Academy of Sciences of the United States of America, 112, pp. 8290 - 8295, http://dx.doi.org/10.1073/pnas.1506538112
, 2015, 'Structure of the receptor-binding carboxy-terminal domain of the bacteriophage T5 L-shaped tail fibre with and without its intra-molecular chaperone', Viruses, 7, pp. 6424 - 6440, http://dx.doi.org/10.3390/v7122946
, 2015, 'Generation of monoclonal antibodies specific of the postfusion conformation of the Pneumovirinae fusion (F) protein', Journal of Virological Methods, 224, pp. 1 - 8, http://dx.doi.org/10.1016/j.jviromet.2015.08.002
, 2015, 'Intracellular expression of Tat alters mitochondrial functions in T cells: A potential mechanism to understand mitochondrial damage during HIV-1 replication', Retrovirology, 12, http://dx.doi.org/10.1186/s12977-015-0203-3
, 2015, 'First report of Babesia divergens infection in an HIV patient', International Journal of Infectious Diseases, 33, pp. e202 - e204, http://dx.doi.org/10.1016/j.ijid.2015.02.005
, 2014, 'New Insights into Rotavirus Entry Machinery: Stabilization of Rotavirus Spike Conformation Is Independent of Trypsin Cleavage', PLoS Pathogens, 10, http://dx.doi.org/10.1371/journal.ppat.1004157
, 2014, 'Self-assembly and characterization of small and monodisperse dye nanospheres in a protein cage', Chemical Science, 5, pp. 575 - 581, http://dx.doi.org/10.1039/c3sc52276h
, 2014, 'Cryo-EM near-atomic structure of a dsRNA fungal virus shows ancient structural motifs preserved in the dsRNA viral lineage', Proceedings of the National Academy of Sciences of the United States of America, 111, pp. 7641 - 7646, http://dx.doi.org/10.1073/pnas.1404330111
, 2014, 'Severe babesiosis in immunocompetent man, Spain, 2011', Emerging Infectious Diseases, 20, pp. 724 - 726, http://dx.doi.org/10.3201/eid2004.131409
, 2014, 'Polyclonal and monoclonal antibodies specific for the six-helix bundle of the human respiratory syncytial virus fusion glycoprotein as probes of the protein post-fusion conformation', Virology, 460-461, pp. 119 - 127, http://dx.doi.org/10.1016/j.virol.2014.05.001
, 2013, 'Uncoating of common cold virus is preceded by RNA switching as determined by X-ray and cryo-EM analyses of the subviral A-particle', Proceedings of the National Academy of Sciences of the United States of America, 110, pp. 20063 - 20068, http://dx.doi.org/10.1073/pnas.1312128110
, 2013, 'Crystallization of the C-terminal domain of the bacteriophage T5 L-shaped fibre', Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 69, pp. 1363 - 1367, http://dx.doi.org/10.1107/S1744309113028959
, 2012, 'Neutralizing antibodies against the preactive form of respiratory syncytial virus fusion protein offer unique possibilities for clinical intervention', Proceedings of the National Academy of Sciences of the United States of America, 109, pp. 3089 - 3094, http://dx.doi.org/10.1073/pnas.1115941109
, 2012, 'Cryphonectria nitschkei virus 1 structure shows that the capsid protein of chrysoviruses is a duplicated helix-rich fold conserved in fungal double-stranded RNA viruses', Journal of Virology, 86, pp. 8314 - 8318, http://dx.doi.org/10.1128/JVI.00802-12
, 2012, 'Epitope insertion at the N-terminal molecular switch of the rabbit hemorrhagic disease virus T=3 capsid protein leads to larger T=4 capsids', Journal of Virology, 86, pp. 6470 - 6480, http://dx.doi.org/10.1128/JVI.07050-11
, 2012, 'Insights into minor group rhinovirus uncoating: The X-ray structure of the HRV2 empty capsid', PLoS Pathogens, 8, http://dx.doi.org/10.1371/journal.ppat.1002473
, 2011, 'Molecular rearrangements involved in the capsid shell maturation of bacteriophage T7', Journal of Biological Chemistry, 286, pp. 234 - 242, http://dx.doi.org/10.1074/jbc.M110.187211
, 2010, 'The T=1 capsid protein of Penicillium chrysogenum virus is formed by a repeated helix-rich core indicative of gene duplication', Journal of Virology, 84, pp. 7256 - 7266, http://dx.doi.org/10.1128/JVI.00432-10
, 2010, 'Electrostatic interactions between capsid and scaffolding proteins mediate the structural polymorphism of a double-stranded RNA virus', Journal of Biological Chemistry, 285, pp. 3643 - 3650, http://dx.doi.org/10.1074/jbc.M109.075994
, 2009, 'The mechanism of vault opening from the high resolution structure of the N-terminal repeats of MVP', EMBO Journal, 28, pp. 3450 - 3457, http://dx.doi.org/10.1038/emboj.2009.274
, 2009, 'The capsid protein of infectious bursal disease virus contains a functional α4β1 integrin ligand motif', Virology, 386, pp. 360 - 372, http://dx.doi.org/10.1016/j.virol.2008.12.036
, 2009, 'Infectious Bursal Disease Virus: Ribonucleoprotein Complexes of a Double-Stranded RNA Virus', Journal of Molecular Biology, 386, pp. 891 - 901, http://dx.doi.org/10.1016/j.jmb.2008.11.029
, 2009, 'Infectious bursal disease virus is an icosahedral polyploid dsRNA virus', Proceedings of the National Academy of Sciences of the United States of America, 106, pp. 2148 - 2152, http://dx.doi.org/10.1073/pnas.0808498106
, 2008, 'Sharpening high resolution information in single particle electron cryomicroscopy', Journal of Structural Biology, 164, pp. 170 - 175, http://dx.doi.org/10.1016/j.jsb.2008.05.010
, 2007, 'Infectious bursal disease virus capsid assembly and maturation by structural rearrangements of a transient molecular switch', Journal of Virology, 81, pp. 6869 - 6878, http://dx.doi.org/10.1128/JVI.00077-07
, 2006, 'Three-dimensional structure and stoichiometry of Helmintosporium victoriae190S totivirus', Virology, 347, pp. 323 - 332, http://dx.doi.org/10.1016/j.virol.2005.11.038
, 2005, 'Structural polymorphism of the major capsid protein of a double-stranded RNA virus: An amphipathic α helix as a molecular switch', Structure, 13, pp. 1007 - 1017, http://dx.doi.org/10.1016/j.str.2005.04.012
, 2004, 'The C-terminal domain of the pVP2 precursor is essential for the interaction between VP2 and VP3, the capsid polypeptides of infectious bursal disease virus', Virology, 322, pp. 135 - 142, http://dx.doi.org/10.1016/j.virol.2004.01.025
, 2004, 'UCSF Chimera--a visualization system for exploratory research and analysis.', J Comput Chem, 25, pp. 1605 - 1612, http://dx.doi.org/10.1002/jcc.20084
, 2003, 'Three-dimensional structure of Penicillium chrysogenum virus: A double-stranded RNA virus with a genuine T=1 capsid', Journal of Molecular Biology, 331, pp. 417 - 431, http://dx.doi.org/10.1016/S0022-2836(03)00695-8
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Location
Electron Microscope Unit
Mark Wainwright Analytical Centre
Chemical Sciences Building (F10) &
School of Biomedical Sciences,
Faculty of Medicine & Health
Room 228, Level 2, Wallace Wurth Building (C27)
Kensington UNSW Sydney
NSW 2052
Mark Wainwright Analytical Centre
Chemical Sciences Building (F10) &
School of Biomedical Sciences,
Faculty of Medicine & Health
Room 228, Level 2, Wallace Wurth Building (C27)
Kensington UNSW Sydney
NSW 2052
