Fluorescence 3D Neuron Tracing
The Zeiss Axio Imager M2 upright microscope with ApoTome 2 with MBF Bioscience software: Stereo Investigator and Neurolucida. This system can be used for neuronal tracing, morphometric quantification, and unbiased stereology (number, length, area and volume of cells and biological structure stereology of histological specimens) in both brightfield and fluorescence. The system is fitted with 6 objectives ranging from 2.5x to 63x, making it ideal for widefield imaging of cells or tissues. The Apotome 2 allows optical sectioning of fluorescent samples by the removal out of focus light with structured illumination (uses grid patterns to calculate the optical sections). The system has both monochrome and colour cameras.
