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High-parameter flow cytometry is a powerful tool that enables scientists to identify and analyze distinctive phenotypes in heterogeneous populations. Advances in biology and a greater understanding of immunological structure and function allow us to ask more complex questions that require a comprehensive solution.

Automation Status

Follow@Gyro_NMR_UNSW

Instrument Training Contact Adelle Amoore a.amoore@unsw.edu.au

Currently Operational

Follow@Pines_NMR_UNSW

Instrument Training Contact Aditya Rawal a.rawal@unsw.edu.au

Currently Operational

BCU-2 removed for service

- room temperature up to 80 ^oC experiments only

Automation Status

Follow@Guass_NMR_UNSW

Instrument Training Contact Doug Lawes d.lawes@unsw.edu.au

and Donald Thomas donald.thomas@unsw.edu.au

Instrument Training Contact Doug Lawes d.lawes@unsw.edu.au

and Donald Thomas donald.thomas@unsw.edu.au

The MRI facility at the UNSW’s BRIL is one of the most powerful small-animal MRIs in Australia and is able to provide users with state-of-the-art small animal and sample imaging, and MRI biomarker assessment. As part of the National Imaging Facility the scanner can be accessed by researchers from institutions from all over the country.

The Bruker D8 Venture is the centre-piece of the Structural Biology Facilty.

Currently Operational

Instrument Training Contact

Donald Thomad donald.thomas@unsw.edu.au

The Bruker MultiMode® 8 (MM8) is an advanced atomic force microscope (AFM) and Scanning Tunnelling Microscope (STM). Its PeakForce Tapping technology enables exceedingly low tip-sample force interactions compared to tapping mode, making it particularly useful for delicate samples by allowing the user to control the maximal force imparted to the sample. The MM8 also features the Bruker ScanAsyst® software, automating the optimisation of scanning parameters for users to obtain quality images.

The BD FACSAria™ III cell sorter is equipped with 5 excitation lasers (blue 488nm, red 642nm and violet 405nm + Yellow Green 561nm and True Blue 442nm) and can detect up to 17 parameters (15 fluorescence channels plus forward scatter and side scatter).

The BD FACSMelody™ Cell Sorter with a 2 laser system (488nm and 561nm) with 7 fluorescent detectors and 2 way sorting it is a simple and easy to use sorter. With BD FACSChorus™ Software, the entire cell sorting process is designed to eliminate manual steps, making cell sorting easy to learn, and the BD FACSMelody Sorter easy to use.

The Cytek Aurora cell sorter is equipped with five excitation lasers (ultraviolet 355nm, violet 405nm, blue 488nm, yellow/green 561nm and red 642nm) and has a flexible detection configuration with a number of filter set options, detecting up to 24 parameters (21 fluorescence channels, two forward scatter channels and one side scatter).

The ALS CellCelector is a freely configurable tool for the automated transfer of single cells and cell colonies. The patented harvest process allows a gentle cell uptake directly from the culture plate without pre-treatment. Using this technology, highest survival rate and cell integrity are guaranteed. Our new developed laminar flow cabinet allows you to work permanently under sterilizable and defined environmental conditions (temperature, CO2). A controlled cell harvest has an increasing importance for the applications of the rapidly expanding life science sector.

Specimen Preperation

Chemicon Advanced Tissue Arrayer ATA100 allows you to create customized arrays of multiple tissue specimens on a single histologic slide. This instrument allows to selectively remove core tissue samples from paraffin blocks and arrange those tissue samples into an array within a recipient paraffin block. The newly formed tissue array paraffin block is then ready for further histology technique processing.

Ten PC's available for training and off-line processing.

The AcCellerator is a high-speed imaging cytometer for mechanical cell characterization through real-time deformability cytometry measurements.