Confocal Rapid FLIM Super-resolution Funded by Cancer Institute NSW
Fluorescence Lifetime Imaging (FLIM) adds another dimension to conventional intensity based imaging methods. FLIM is not affected by fluctuations in the fluorescence intensity and permits to discrimination between fluorophores with similar emission spectra (and from auto-fluorescence. It can be used to probe local environmental conditions (e.g., pH value), to determine ion concentrations, to study intracellular signal transduction or to distinguish between different tissue components.