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Image Analysis

We currently have 8 image analysis computers, all of them accessible remotely, whilst 4 are also accessible locally with 4k 48inch displays so you can see your data in its best light. We also have a varied range of commercial software packages available on many of these computers

Fluorescence Phase Contrast Widefield High Throughput

he IncuCyte S3 is a widefield inverted live cell system engineered with long term high throughput imaging in mind. Housed inside a tissue culture incubator, the imaging module maintains the most stable imaging conditions possible ( 37C temperature, humidity and 5% CO2). The system comes with a selection of versatile inserts allowing you to image your cells in a range of vessels.

Fluorescence Phase Contrast Widefield High Throughput

The Incucyte SX5 is engineered for long-term, high throughput, widefield microscopy. The system is compatible with a range of plates and cell culture flasks in stable conditions (37°C temperature, stable humidity and 5% CO2), with automated imaging for long-term experiments.  

Images can be acquired over time and analysed in real time. The SX5 supports a wider range of fluorescent imaging than the S3, with up to five fluorescent channels available. The acquisition software is loaded with a variety of analysis tools and modules, details below.  

The Inveon μCT uses multiple axial x-rays of the animal to generate cross-sectional information or 3-dimensional reconstructions of the animal or parts of the animal. The μPET uses an LSO detector for fast scintillation decay time, high light output and effective atomic number.

Optoacoustic or photoacoustic imaging is a new, cutting-edge imaging technique which detects ultrasound waves following light absorption from specific molecules.  This technique allows molecules with different spectral profile to be detected.

The IVIS SpectrumCT provides a highly sensitive means to image fluorescent and/or bioluminescent reporters in vivo and in vitro, as well as providing a microCT capability for anatomical referencing.

AFM

The NanoWizard® 4 XP atomic force microscope is an advanced Atomic Force Microscope (AFM). AFM is a very high-resolution type of Scanning probe microscopy (SPM) which acquires data by “feeling” or “touching” the surface of a sample with a mechanical probe

Specimen Preparation OPAL

The Bond RXm automated staining system increases reproducibility of IHC (chromogenic or immunofluorescent) and multiplex staining. Custom protocols can be programmed for projects, complete with temperature control for steps such as antigen retrieval or TSA staining. Long staining protocols, such as OPAL multiplex can be set to be completed as an overnight run, while shorter protocols can be run during the business day

Specimen Preperation

The BOND RX provides an easy way to automate the preparation of samples. In combination with PerkinElmer Opal™ Kit it is also useful for multiplexing allowing up to 9 fluorescent labels within a single sample.

Fluorescence Widefield

The Leica DM IL microscope is used to illuminate whole cells; observing and analysing a cell as a single entity. The DM IL is an inverted epifluorescence microscope, with transmitted light LED back illumination and an EL6000 Fluoro system.

Specimen Preperation

Leica RM2135 rotary microtome is used to cut very thin cross-sections of specimens (usually 4 µm) for routine and research histology studies in the fields of biology and medicine.  This instrument is designed for cutting soft and harder paraffin embedded specimens, as long as they are suitable for being cut manually.  The sectioning thickness setting range can be from 1 µm to 60 µm.  High quality paraffin sections could be used for varying staining, such as Haematoxylin and Eosin stain (H&E), immunohistochemistry (IHC), Immunofluorescent staining (IF) and In situ hybridization (ISH).

Confocal Lightsheet

The Leica TCS SP8 DLS (Digital LightSheet) is an inverted laser scanning microscope capable to be used for confocal imaging and light sheet imaging. This microscope is able to image multi-colour, live, fixed or aqueous-based cleared specimens utilising a combinations of 405, 458, 488, 514, 561 and 635 nm excitation lasers through the employment of the AOBS with spectral high quantum efficiency detectors. The advantage of the two combined features (confocal/Lightsheet) on a single equipment allow to perform, with the confocal module, local photo activation or photo bleaching where the resulting dynamics can be capture via lightsheet imaging on thick sample (up to 2 mm thick and 1 cm long).

The MILabs U-CT installed in 2019 is a cutting-edge preclinical micro-CT scanner which has expanded BRIL's imaging capabilities by allowing larger sample sizes and shorter scan times.